Method to treat propolis

ABSTRACT

A method to treat propolis includes conducting at least a first extraction of raw propolis with an aqueous solvent to obtain an aqueous extract having first properties. A second extraction may be performed with an alcohol solvent on the filtration residues obtained after the first extraction. A third extraction may be performed with an oil solvent on the filtration residues obtained after the second extraction. The method applies to the production of extracts of propolis for use in the manufacture of hygiene, care, cosmetic products, food products and food supplements.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a division of application Ser. No. 12/595,149 filed on Jan. 27, 2010 (issued as U.S. Pat. No. 8,257,747); which is the 35 U.S.C. 371 national stage of International application PCT/FR08/50736 filed on Apr. 23, 2008; which claims priority to French application 0754648 filed on Apr. 24, 2007. The entire contents of each of the above-identified applications are hereby incorporated by reference.

FIELD OF THE INVENTION

The invention concerns a method to treat propolis. It also concerns the extracts of propolis obtained with this method.

BACKGROUND OF THE INVENTION

The invention applies to the manufacture of hygiene, care, cosmetic, food and food supplement products, comprising one or more propolis extracts obtained with the method.

It is recalled that propolis designates a whole series of resinous, gum and balsamic substances of viscous consistency. These substances are collected on some parts (essentially the buds and bark) of plants (chiefly certain trees) by bees which carry them back to the hive and partly modify them by adding some of their own secretions (essentially wax and saliva secretions).

Propolis is used for numerous purposes inside the hive:

-   -   possibly to construct true defence barriers;     -   to achieve a perfect seal for the hive, allowing good heat         insulation;     -   to varnish all the inner surfaces to eliminate roughness;     -   to coat new combs and the inside of all the cells with a fine         film before the queen comes to lay eggs therein, amounting to         efficient disinfection (type of “sterilisation”);     -   finally, to coat, together with wax, all the small animals or         insects which cannot be evacuated, a kind of embalming to         prevent any putrid decomposition.

It is recognized by the scientific community that propolis is a substance having the following properties:

-   -   major antibiotic properties extending to numerous microbial         strains, in addition propolis extract increases (potentializes)         the efficacy of some antibiotics. Further, experiments have         shown that strains of pathogenic microbes are much more         sensitive to the action of propolis than of conventional         antibiotics: penicillin, tetracycline, ampicillin, monomycin,     -   antiviral,     -   antifungal,     -   anti-germinating,     -   very powerful anaesthetic properties, much greater inter alia         than that of cocaine and additionally does not have its         disadvantages with regard to side effects,     -   notable healing properties through the stimulation of tissue         regeneration,     -   action on neuron metabolism,     -   antioxidant,         to which must be further added non-negligible anti-inflammatory         properties and a favourable influence on some immunological         mechanisms which leads to strengthening of predisposition         against aggressions in general.

Numerous presentations currently exist, of which many in the form of preparations in which propolis is:

-   -   either the only active ingredient, in its purified form (i.e.         rid of all various mechanical impurities which may have tainted         it in the hive: wood fibres, bee hairs, etc.);     -   or in association (again in its purified form) with other         products which are generally medicinal or dietary products.

Therefore, to conclude, at the present time the following is most often found:

-   -   propolis in the natural state, purified and the only active         ingredient in the form of:     -   a solid: chewing gum or fragments (of variable size), granules         and powder (the latter possibly being in capsules or tablets to         be swallowed),     -   an extract diluted in alcohol: hydroalcoholic solution of         propolis in varying concentrations (3 to 30% depending on         indications, the average being 15 to 20%),     -   purified propolis in association with:     -   various medicinal substances which complete the action of         propolis in some particular indications,     -   ointment or unguent, in which propolis is generally associated         with vaseline or lanoline (or both together) in varying         percentages generally ranging from 10 to 30% (average of 15%),     -   dietary products which are most often: honey, pollen or royal         jelly or non-apical products (plants),     -   cosmetics.

SUMMARY OF THE INVENTION

The subject of the present invention is a natural method to treat propolis with which it is possible to obtain a novel presentation of propolis in the form of an extract maintaining all the active components of raw propolis, no solvent mixed with another solvent or with any another product being used for extraction.

A more particular subject of the invention is a natural method to treat propolis, solely using pure biological solvents compatible with organic certification. This method provides several types of extracts, easily soluble and mixable in any type of formula (oil, water, alcohol). The method therefore allows multiple possibilities of application (creams, balsams, dietary preparations), all the types of extract being used as ingredients in comestible products and able to be applied by cutaneous route and ingested by oral route or nasal route.

More particularly, the invention concerns a method to treat propolis consisting of performing at least one first extraction of propolis on a batch of raw propolis with a first pure solvent, to obtain a first extract having first properties.

The method may comprise at least a second extraction performed with a second pure solvent of different type to the first, the extraction with the second solvent being performed on the filtration residues obtained after the extraction performed with the first solvent, so as to collect and make usable all the propolis components, in addition only using organic solvents; and finally, the method may comprise a third extraction following after the second, performed on the filtration residues obtained after this second extraction using a third pure solvent different from the two others; the first solvent then being water, the second being alcohol and the third being oil.

The invention applies to the production of propolis extracts for use in particular in the manufacture of hygiene, care and cosmetic products, food and food supplements.

Therefore, according to a first characteristic, the method to treat propolis comprises an extraction step with a first pure solvent i.e. not mixed with another solvent, performed on a batch of raw propolis to obtain a first extract having first properties.

If the first solvent is water, the extract obtained is an aqueous extract whose properties are antibacterial and isotonic properties i.e. compatible with application to mucosa and applicable directly onto the skin.

According to another characteristic, the extraction of propolis with water is conducted by decoction of propolis, by placing the propolis in water and bringing it to the boil and/or by infusion leaving the propolis to stand in water which has been brought to the boil. Contrary to preconceived opinion, an extract is thereby obtained which has strong activity, the active matter of propolis being maintained.

If the first solvent is alcohol, the extract obtained is an alcohol extract whose properties are antiseptic, antibacterial properties, able to be used in the manufacture of any hygiene or cosmetic product.

Alcohol extraction comprises the following steps:

-   -   5.1—Raw propolis is left to macerate and is manually mixed         discontinuously for at least 15 days,     -   5.2—After one or more filtrations, the filtration residues are         collected,     -   5.3—The alcohol extract obtained is collected.

According to another characteristic of the invention, the method comprises a second extraction step with a second pure solvent of different type to the first; extraction with the first solvent being performed on the batch of raw propolis and extraction with the second solvent being conducted on the filtration residues obtained after the extraction conducted with the first solvent, so as to obtain the first propolis extract having first properties then a second extract having second properties, the latter being rid of most impurities.

To obtain a first extract of propolis having isotonic properties i.e. compatible with application to the mucosa (nasal, oral, vaginal) and which can be applied directly to the skin or is able to be consumed, the first solvent is water, the first extract being an aqueous extract, and the second solvent is alcohol, the second extract being an alcohol extract of greater purity (fewer impurities) than existing alcohol extracts.

Alcohol extraction then comprises the following steps:

-   -   1—The filtration residues derived from the aqueous extraction         step are macerated in alcohol and manually mixed discontinuously         for at least 15 days,     -   2—After one or more filtrations, the filtration residues are         collected,     -   3—The alcohol extract obtained is collected.

For other particular applications, creams, balsams, dietary preparations, therapeutic preparations, the first solvent is alcohol, the first extract being an alcohol extract, the second solvent is oil, the second extract being an oil extract.

In one particularly advantageous embodiment, since it allows a total extract to be obtained i.e. an extract not containing any impurity and containing 100% of the active components of propolis, at least three successive extractions are conducted using solvents of different type, the first solvent is water, the first extract obtained being an aqueous extract, the second solvent is alcohol, the second extract obtained being an alcohol extract, and the third solvent is oil, the third extract being an oil extract, the first extraction being performed on a first batch of propolis and the successive extractions each being performed on the filtration residues obtained after the preceding extraction. With the method, it is therefore possible to collect and to make usable the entirety of the propolis components.

With the treatments of propolis using water as first solvent, and in order to ensure good penetration of the water into the raw propolis, the first step of the method consists of holding the propolis at a temperature of less than zero degrees for a relatively long period, for example 30 days.

The following step is a decoction step of the propolis. The propolis is placed in water brought to the boil and whose temperature is held for sufficiently long time but preferably not exceeding 20 minutes, and the mixture is then left to stand and cool.

Leaving the mixture to stand allows separation of the wax contained in the propolis, and the extract can be collected after one or more filtrations.

The filtration residues are kept for the following extraction(s).

The second extraction comprises maceration in alcohol and daily, discontinuous, manual mixing of the filtration residues obtained after the first extraction for a period of at least fifteen days, followed by one or more filtrations of the extract to obtain the purified alcohol extract and filtration residues.

If three successive extractions are performed, allowing a total extract to be obtained,

-   -   the first extraction consists of:         -   Conducting long, aqueous extraction of propolis to obtain an             aqueous extract of propolis and filtration residues, holding             the propolis at a temperature of less than zero degrees for             a relatively long period, for example 30 days, then carrying             out decoction of the propolis, the propolis being placed in             water brought to the boil for a time not exceeding 20             minutes, and finally leaving it to stand for at least 24 h,     -   the second extraction consists of:         -   Conducting long, alcohol extraction using the filtration             residues obtained after the aqueous extraction, to obtain an             alcohol extract of propolis and filtration residues, by             macerating the filtration residues in alcohol and mixing             manually and discontinuously for at least 15 days,     -   the third extraction consists of:         -   Conducting long, oil extraction using the residues from the             alcohol extraction to obtain an oil extract of propolis and             filtration residues, by leaving the residues from alcohol             extraction to macerate in oil for at least one week.

The invention applies to the manufacture of hygiene, health, cosmetic, food and food supplement products.

The invention applies to the manufacture of a body deodorant comprising at least 50% of aqueous extract of propolis in its composition, obtained following the method of the present invention.

The invention applies to the manufacture of a nasal hygiene product containing 50% of aqueous extract of propolis in its composition, obtained following the method of the present invention.

The invention applies to the manufacture of an ear hygiene product consisting of 100% aqueous extract of propolis obtained following the method of the present invention.

The invention applies to the manufacture of an intimate hygiene product containing 5% alcohol extract of propolis in its composition, obtained after a second extraction according to the method.

The invention applies to the manufacture of an after-shave care cream comprising a complex of propolis extracts containing 14% oil extract and 10% aqueous extract in its composition obtained following the method.

The invention applies to the manufacture of an anti-oxidant product for the area around the eyes, having a smoothing effect on eyebags and fine lines, comprising a complex of propolis extracts containing 14% oil extract and 10% aqueous extract in its composition obtained using the method of the invention.

The invention applies to the manufacture of an anti-oxidant product for body care having a hydrating and protective effect, comprising a complex of propolis extracts containing 8% propolis oil extract and 12% propolis aqueous extract in its composition, obtained using the method of the invention.

The invention applies to the manufacture of a shower cream for the body, face and hair, containing 1% alcohol extract in its composition obtained following the method according to the invention.

The invention applies to the manufacture of a regenerating care shampoo, containing 2.5% alcohol extract in its composition, obtained using the method according to the invention.

The invention applies to the manufacture of a toothpaste, containing at least 3% alcohol extract of propolis in its composition, obtained using the method according to the invention.

BRIEF DESCRIPTION OF THE DRAWINGS

Other particular aspects and advantages of the invention will become clearly apparent on reading the description given below as a non-limiting, illustrative example with reference to the figures in which:

FIG. 1 gives the steps performed according to the method of the invention in a first embodiment,

FIG. 2 gives the steps performed according to the method of the invention in a second embodiment,

FIG. 3 gives the steps performed according to the method of the invention in a third embodiment,

FIG. 4 gives the detailed steps of an aqueous extraction according to the invention,

FIG. 5 gives the steps of an alcohol extraction according to the invention,

FIG. 6 gives the steps of an oil extraction according to the invention.

DETAILED DESCRIPTION OF THE INVENTION

With the method of the invention, it is possible to obtain two or three extracts of different type (aqueous, alcohol and oil) which may be used for different applications.

With the method, it is further possible, after each extraction, to obtain different active components of propolis. The advantage provided by extraction with water followed by extraction with alcohol, is that water extraction only removes active materials that are non-soluble in alcohol, and water vapour facilitates the dissolving in alcohol of alcohol-soluble active components. Similarly, by performing extraction with alcohol and then with oil, no impurity is obtained (100% purity) and 100% active components.

For this purpose, the method comprises the conducting of one or two or three successive extractions performed on one same batch of raw propolis (raw propolis for the first extraction) and on the residues obtained after the preceding extractions of propolis for the other one or two extractions. If several extractions of propolis are conducted in sequence, they are not performed on separate batches.

Also, the extractions performed are long extractions (long in time, or slow) as compared with current practice which consists of conducting an extraction within a few hours. This slow conducting and the absence of machines allows the subtle elements to mutate better and avoids deterioration of the physical structure of propolis.

According to a first characteristic of the invention, the method consists of performing an extraction with a first pure solvent i.e. a solvent not mixed with another solvent. This extraction is performed on a batch of raw propolis to obtain a first extract having first properties.

For particular applications, the method goes against preconceived ideas since the solvent is water without any other solvent or additive. With the method, it is therefore possible to obtain an aqueous extract which has the active materials of propolis, imparting to it antiseptic, antibacterial and isotonic properties. The applications are for example the manufacture of deodorants, nasal or ear hygiene products.

The aqueous extract is conducted by decoction of the propolis. The propolis is placed in water and it is brought to the boil. This extract can be obtained by infusing i.e. by leaving the propolis to stand in water which has been brought to the boil. It is also possible to conduct decoction and then to leave the propolis to stand in water brought to the boil (infusion).

For other applications, the pure solvent is alcohol; the extract obtained is an alcohol extract whose properties are antiseptic, antibacterial properties.

According to another characteristic of the invention, the method comprises a second extraction of propolis with a second pure solvent of different type to the first. Extraction with the first solvent is performed on the batch of raw propolis, and extraction with the second solvent is performed on the filtration residues obtained after the extraction with the first solvent, so as to obtain the first extract of propolis having first properties then a second extract having second properties, this latter being rid of most of the impurities.

In a first embodiment, the first solvent is water and the second solvent is alcohol.

In this first embodiment allowing an aqueous extract and an alcohol extract to be obtained from one batch of propolis, the following operations are carried out:

A] Long, Aqueous Extraction:

-   -   1—The propolis is held at a temperature of less than zero         degrees for a relatively long period, for example 30 days,     -   2—Decoction of the propolis: the propolis is placed in water and         brought to the boil for a time not exceeding 20 minutes, then it         is left to stand for at least 24 h,     -   3—The extract is collected. This collection is made after one or         more filtrations.     -   4—The filtration residues are collected.

B] Long, Alcohol Extraction: The residues obtained after step 4 are used to perform alcohol extraction according to the scheme in FIG. 5.

-   -   5—The filtration residues are left to macerate in alcohol, and         are mixed manually and discontinuously for at least 15 days,     -   6—The filtration residues are collected after one or more         filtrations,     -   7—The alcohol extract obtained is collected.

Second Embodiment: treatment of a batch of propolis, the first solvent is alcohol and the second solvent is oil.

B′] Long, alcohol extraction: Steps 5 to 7 just described are implemented, however the treatment is started with raw propolis, step 5 is therefore performed on raw propolis and not on filtration residues.

-   -   5.1—The raw propolis is left to macerate and is manually mixed         discontinuously for at least 15 days,     -   5.2—After one or more filtrations, the filtration residues are         collected,     -   5.3—The alcohol extract obtained is collected.

C] Oil Extraction:

-   -   8—The filtration residues collected after step 5.2 are left to         macerate in food oil held at constant temperature for at least         one week, preferably for one to four weeks,     -   9—Filtering or decanting is performed,     -   10—The oil extract obtained is collected.

According to another characteristic of the method, three successive extractions are performed.

In this case, the successive conducting is performed of an aqueous extraction, then an alcohol extraction, then an oil extraction using one same batch of raw propolis.

In one particular embodiment, the following steps are carried out:

A) Long, Aqueous Extraction (FIG. 4):

-   -   1—Holding the raw propolis at a temperature of less than zero         degrees for a relatively long time, for example 30 days,     -   2—Decoction of the propolis: the propolis is placed in water and         brought to the boil for a time not exceeding 20 minutes,     -   3—This is left to stand for at least 24 h to collect the         extract. This collection is conducted after one or more         filtrations,     -   4—The filtration residues are collected.

B) Long, Alcohol Extraction (FIG. 5):

-   -   The residues derived from step 4 are used to perform alcohol         extraction following the scheme in FIG. 5.     -   5—The filtration residues are left to macerate in alcohol and         are manually mixed discontinuously for at least 15 days,     -   6—After one or more filtrations, the filtration residues are         collected,     -   7—The alcohol extract obtained is collected.

C] Oil Extraction (FIG. 6):

-   -   8—The filtration residues collected after step 6 are left to         macerate in oil,     -   9—Filtration or decanting is conducted,     -   10—The oil extract obtained is collected.

With the present invention, it is therefore possible to obtain an aqueous extract, an alcohol extract and an oil extract—each extract can be used independently or associated with another for the production of hygiene, health or cosmetic products, food products or food supplements.

Some examples of application are given below. These applications were the subject of laboratory tests confirming the stated properties and tolerance by the human body.

Applications of extracts of propolis and of complexes of propolis extracts obtained following the method described above:

1—Body Deodorant (Armpits, Chest, Moist Feet and Hands, and Intimate Areas) which the Applicant Calls: DEODORANT UNIVERSEL BIS.

Composition: 99.9% aqueous extract of organic propolis and 0.1% elixir marketed by the applicant under the name Elixir de la Ruche Sueur du Ciel.

Different studies were carried out by Institut Dermatologique d'Aquitaine on the above formula, targeting the evaluation of:

a—cosmetic acceptability

b—gynaecological tolerance under the supervision of a gynaecologist,

c—Skin tolerance under the supervision of a dermatologist.

Conclusions of the study report show that:

The formula has:

-   -   good global cutaneous tolerance;     -   satisfactory cosmetic acceptability;     -   good skin-mucosal tolerance.

d—bactericidal action on the strains:

d1—Corynebacterium xerosis

d2—Staphylococcus epidermidis

d3—Propionibacterium acnes

2—Intimate Hygiene with High Tolerance, which the Applicant Calls: PAIN HYGIÈNE INTIME,

Composition: 94% organic soap batter of plant origin (commercially available), organic honey (commercially available), 5% alcohol extract of organic propolis obtained following the method, 1% elixir marketed by the applicant under the name Elixir de la Ruche Vol Nuptial.

Different studies were carried out by Institut Dermatologique d'Aquitaine on the above formula.

a—Evaluation of skin tolerance after a single application covered by an occlusive dressing for 48 hours under the supervision of a dermatologist.

Conclusion of the study report: the PAIN HYGIÈNE INTIME applied in a 2% solution can be considered to be non irritant after 48 hours' consecutive application under an occlusive dressing in 11 volunteers.

b—Evaluation of gynaecological tolerance and of cosmetic qualities after 21 days' application under the supervision of a gynaecologist.

Conclusions of the study report: the PAIN HYIÈNE INTIME has good global skin-mucosal tolerance and good cosmetic acceptability.

3—Soothing After-Shave Care—Micro-Cuts—Natural Healing

Product called: CRÈME DE FORCE by the applicant.

Composition: emulsion (76% aqueous or oil base) prepared with a complex of propolis extracts: 14% oil extract and 10% aqueous extract of propolis.

Different studies are in progress by Institut Dermatologique d'Aquitaine on the above formula with a view to confirming:

-   -   The above-cited properties (soothing after-shave         care—micro-cuts—natural healing).     -   skin tolerance after a single application under an occlusive         dressing for 48 hours.     -   skin tolerance and cosmetic qualities after 21 days of         application.     -   Eye tolerance.

4—Antioxidant—Smoothes Eye Bags and Fine Lines

Product called: YEUX DE REINE by the applicant.

Composition: emulsion (76% aqueous or oil base) prepared with a complex of propolis extracts: 14% oil extract and 10% aqueous extract of propolis.

-   -   The above-cited properties (antioxidant—smoothes eye bags and         fine lines).     -   Skin tolerance after a single application under an occlusive         dressing for 48 hours.     -   Skin tolerance and cosmetic qualities after 21 days'         application.     -   Eye tolerance.

5—Antioxidant—Hydrating and Protective

Product called: LAIT DE RUCHE by the applicant.

Composition: emulsion (80% aqueous or oil base) prepared with a complex of propolis extracts: 8% oil extract and 12% aqueous extract of propolis.

Different studies are in progress by Institut Dermatologique d'Aquitaine on the above formulas with a view to confirming:

-   -   The above-cited properties (antioxidant—hydration—protection).     -   Skin tolerance after a single application under an occlusive         dressing for 48 hours,     -   Skin tolerance and cosmetic qualities after 21 days'         application.

6—Healthy Scalp

-   -   Itchy scalp,     -   Scalp cleansing,     -   Anti-dandruff,     -   Anti-hair loss—alopecia     -   →for healthy reinvigorated hair.

Product called: CRÈME DOUCHE DE LA RUCHE by the applicant

Composition: 94% cleansing base of plant origin, 1% alcohol extract of propolis, 5% honey.

Product called: SHAMPOING DOUCHE ASSAINISSANT ET DOUX by the applicant.

Composition: 94% cleansing base of plant origin, 1% alcohol extract of propolis, 5% honey

Product called: SHAMPOING SOIN RÉGÉNÉRANT by the applicant.

Composition:97.5% cleansing base of plant origin, 2.5% alcohol extract of propolis

7—Nose and Ear Hygiene

Nasal Application

-   -   Allergies and seasonal sensitivity—antiviral action     -   Cleansing of pollution residues     -   Hydration of nasal mucosa.

Product called: SPRAY NASAL DES PYRÉNÉES by the applicant.

Composition: isotonic preparation containing 50% plant extracts and 50% aqueous extract of propolis.

Ear Application

-   -   Soothing, cleansing, anti-itch ear bath.

Product called EXTRAIT DE PROPOLIS SANS ALCOOL by the applicant (alcohol-free extract).

Composition: isotonic preparation containing 100% aqueous extract of propolis.

8—Dental and Mouth Hygiene

-   -   Natural whitening through anti-bacterial effect,     -   Parodontosis,     -   Cleansing of contours of dental implants and prostheses. Product         called: DENTIFRICE SOURIRE by the applicant.

Composition: preparation containing 3.1% alcohol extract of propolis and 96.9% of a basic hygiene product for teeth, available commercially.

Different studies have been conducted by Institut Dermatologique d'Aquitaine on the above formula, including evaluation of tolerance and acceptability after 21 days' application under the supervision of a stomatologist.

Conclusions of the study report: the formula shows very good stomatological tolerance and good cosmetic acceptability. 

The invention claimed is:
 1. A cosmetic product, comprising a composition of at least one of: an aqueous extract of propolis, an alcohol extract of propolis or an oil extract of propolis, wherein the aqueous extract, alcohol extract and oil extract have been obtained by successive extractions of propolis and residue according the following steps: a) an aqueous extraction comprising extracting raw propolis with a pure water solvent and filtering to obtain (i) a first filtration residue and (ii) an aqueous extract having antibacterial, antiseptic and isotonic properties that are compatible with application to mucosa and directly applicable to the skin; b) an alcohol extraction comprising extracting the first filtration residue with a pure alcohol solvent and filtering to obtain (iii) a second filtration residue and (iv) an alcohol extract having antiseptic and antibacterial properties; and c) an oil extraction comprising extracting the second filtration residue with a pure oil solvent and filtering to obtain (v) an oil extract, wherein the product is an aftershave care cream comprising: the oil extract obtained in step c) in an amount of 14% of the composition, and the aqueous extract obtained in step a) in an amount of 10% of the composition.
 2. A cosmetic product comprising a composition of at least one of: an aqueous extract of propolis, an alcohol extract of propolis or an oil extract of propolis, wherein the aqueous extract, alcohol extract and oil extract have been obtained by successive extractions of propolis and residue according the following steps: a) an aqueous extraction comprising extracting raw propolis with a pure water solvent and filtering to obtain (i) a first filtration residue and (ii) an aqueous extract having antibacterial, antiseptic and isotonic properties that are compatible with application to mucosa and directly applicable to the skin; b) an alcohol extraction comprising extracting the first filtration residue with a pure alcohol solvent and filtering to obtain (iii) a second filtration residue and (iv) an alcohol extract having antiseptic and antibacterial properties; and c) an oil extraction comprising extracting the second filtration residue with a pure oil solvent and filtering to obtain (v) an oil extract, wherein the product is an antioxidant product for a surrounding area of the eyes and having an eye bag and fine line smoothing effect, the product comprising: the oil extract obtained in step c) in an amount of 14% of the composition, and the aqueous extract obtained in step a) in an amount of 10% of the composition.
 3. A cosmetic product, comprising a composition of at least one of: an aqueous extract of propolis, an alcohol extract of propolis or an oil extract of propolis, wherein the aqueous extract, alcohol extract and oil extract have been obtained by successive extractions of propolis and residue according the following steps: a) an aqueous extraction comprising extracting raw propolis with a pure water solvent and filtering to obtain (i) a first filtration residue and (ii) an aqueous extract having antibacterial, antiseptic and isotonic properties that are compatible with application to mucosa and directly applicable to the skin; b) an alcohol extraction comprising extracting the first filtration residue with a pure alcohol solvent and filtering to obtain (iii) a second filtration residue and (iv) an alcohol extract having antiseptic and antibacterial properties; and c) an oil extraction comprising extracting the second filtration residue with a pure oil solvent and filtering to obtain (v) an oil extract, wherein the product is an antioxidant product for the body and having a hydrating and protective effect, the product comprising: the oil extract obtained in step c) in an amount of 8% of the composition, and the aqueous extract obtained in step a) in an amount of 12% of the composition. 